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| Product Name: |
TRIzen 每 RNA Extraction Kit
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| Related proudcts |
cost-effective, |
| Specifications |
100ml |
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TRIzenTM Reagent is a ready-to-use reagent, that is used for the isolation of high quality total RNA from a variety of cell and tissue types (cell, animal, plant, yeast, or bacterial origin).
TRIzenTM Reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components which facilitate the isolation of a variety of RNA species of large or small molecular size.
TRIzenTM Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. After homogenizing the sample with TRIzenTM Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation.
RNA isolated with TRIzenTM Reagent can be used for many downstream applications including:
&尚8226; Northern blot and dot blot analysis
&尚8226; RNase protection assays
&尚8226; Molecular cloning &尚8226; mRNA isolation
&尚8226; RT/PCR - In some situations, DNAse treatment is recommended prior to PCR
Featuresㄩ
♂Permits the isolation of RNA, DNA, and protein from the same sample
♂Offers superior lysis capability, even with difficult sample types
♂Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
♂Cost-effective co***red to our competitors. |
| Company: |
Engreen Biosystem Co,Ltd
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| Contact: |
Mr. Jack Yu |
| Address: |
Shop 1, 69 Roseberry Ave., Birkenhead, Auckland, New Zealand. |
| Postcode: |
999030 |
| Tel: |
+64 9 480 8508 |
| Fax: |
+64 9 480 8508 |
| E-mail: |
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